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Evaluating the Effectiveness of VEGF Gene Therapy of Canine Brain Infarction by MR-DWI<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />
刘怀军 武柏林 汪国石 王藏海 王立新 玉鉴 屈长强 池琛 黄勃源
河北医科大学第二医院放射科，河北 石家庄 050000
LIU Huai-jun, WU Bai-lin, WANG Guo-shi, WANG Cang-hai, WANG Li-xin, YU Jian, QU Chang-qiang, CHI Chen, HUANG Bo-yuan
Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
Abstract
Objective：To evaluate the therapeutic effect of VEGF plasmid in cerebral infarction of dog by MR-DWI.
Methods：18 adult dogs were divided into three groups randomly after the focal cerebral ischemic infarction model was produced. Each group was divided into two subgroups,the experimental group was given VEGF plasmid 600μg and the control group was given the same amount of saline. MR scan was performed repeat－edly. After various survival times, specimens were selected and stained with HE or immunohistochemistry. The microblood vessel counts, VEGF im－munostained cells and ADCr were calculated and analyzed by SPSS 10.0.
Results：The signal intensity ofinfarcted tissue on DW images was markedly higher than that of normal brain tissue after stroke, ADC value declined to ( 5.61 ±1.39) mm 2/s,only 43 % of the contralateral. The ADCr of the two groups were significantly different at the end of two weeks ( F =0.032,Ρ= 0.009)； there was significant positive correlation relationship between ADCr and microvessel counts (MVC) ( r = 0.679，Ρ <0. 01).
Conclusion：The application of VEGF plasmid in cerebral infarction of dog can improve the collateral vessel formation； MR -DWI combined with traditional MR sequences can assess the therapeutic effect of VEGF plasmid quantitatively or semiquantitatively.
Key words：magnetic resonance imaging； cerebral infarction； model, animal； vascular endothelial growth factor | 
