您当前的位置:首页 > 核医学

地塞米松对大鼠胸主动脉平滑肌细胞增殖作用的实验研究

时间:2006-08-21 17:09:38  来源:  作者:

 <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

Experimental study about dexamethasone to the proliferation of Rat aortic smooth muscle cells

王建波1,杨建勇1,陈伟1,庄文权1,张泷娟2

Jianbo Wang1,Jianyong Yang1,Wei Chen1,Wenquan Zhuang1,Longjuan Zhang2

1.Department of Interventional Radiology,2.Laboratory of Surgery,The First Affiliated Hospital,SUN Yat-sen University,Guangzhou 510080,China)

(中山大学第一附属医院  1 放射介入科;2 外科实验室 510080)

 

Abstract

  Objective:To evaluate the effect of dexamethasone to the cultured Rat thoracic aortic smooth muscle cells(SMC) in vitro.

  Method:The Rat thoracic aortic SMC were harvest and cultured for six to ten passages. Cultured SMC were synchronized,then restimulated to enter the cell cycle,and treated with incremental concentrations of dexamethasone or without dexamethasone as contrast. The proliferative assay was performed with MTT method in the different time points after treated. DNA synthesis was determined 48 hours after restimulation by measuring [3H] thymidine incorporation. RT-PCR was performed to assay the level of proliferating cell nuclear antigen (PCNA) mRNA.

来顶一下
返回首页
返回首页
 
推荐资讯
掌间筑未来,西门子医疗推出全新CT产品SOMATOM go.
掌间筑未来,西门子医疗
“智”动创新 共享“健康中国” 西门子医疗亮相2017 HOSPEQ
“智”动创新 共享“
西门子医疗携一系列创新成果亮相CMEF 2017   助力“智慧”医疗服务建设
西门子医疗携一系列创
西门子医疗中国亮相第77届中国国际医疗器械春季博览会,多方位展示互联网时代下 “智慧”成果
西门子医疗中国亮相第
最后更新
热门点击
  1. 21世纪核医学需要什么样的技术人员?
  2. 渗透压对对比剂肾病发生率的影响:有关在高
  3. 核医学技师出路在何方?对核医学技术人员队伍
  4. PET-CT在肾上腺肿瘤诊断中的诊断效能和判读
  5. 核医学:传统经典的新兴学科
  6. 冠状动脉造影、心电图、核素检查对冠心病诊
  7. 核医学检查对人体有伤害吗?
  8. 《走进核医学》教学录相片的制作
  9. 高分辨率 MRI 可确诊大脑前动脉夹层
  10. 内皮抑素基因联合放射治疗对大鼠Walker-256